Developmental and cell biological functions of the Drosophila DEAD-box protein Abstrakt

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Developmental and cell biological functions of the Drosophila DEAD-box protein Abstrakt

BACKGROUND DEAD-box proteins are a large family of proteins found in bacteria, plants and animals, but only few have been analysed functionally. They are involved in the regulation of various aspects of RNA processing and metabolism, including splicing, transport and translation. The study of their function in multicellular organisms has been restricted to a few special cases, such as the Vasa ...

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The Drosophila gene abstrakt, required for visual system development, encodes a putative RNA helicase of the DEAD box protein family

The molecular mechanisms underlying axonal pathfinding are not well understood. In a genetic screen for mutations affecting the projection of the larval optic nerve we isolated the abstrakt locus. abstrakt is required for pathfinding of the larval optic nerve, and it also affects development in both the adult visual system and the embryonic CNS. Here we report the molecular characterization of ...

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Abstrakt, a DEAD Box Protein, Regulates Insc Levels and Asymmetric Division of Neural and Mesodermal Progenitors

Asymmetric cell division generates cell diversity in bacteria, yeast, and higher eukaryotes. In Drosophila, both neural and muscle progenitors divide asymmetrically. In these cells the Inscuteable (Insc) protein complex coordinates cell polarity and spindle orientation. Abstrakt (Abs) is a DEAD-box protein that regulates aspects of cell polarity in oocytes and embryos. We use a conditional alle...

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Structural Basis for RNA Unwinding by the DEAD-Box Protein Drosophila Vasa

DEAD-box RNA helicases, which regulate various processes involving RNA, have two RecA-like domains as a catalytic core to alter higher-order RNA structures. We determined the 2.2 A resolution structure of the core of the Drosophila DEAD-box protein Vasa in complex with a single-stranded RNA and an ATP analog. The ATP analog intensively interacts with both of the domains, thereby bringing them i...

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ژورنال

عنوان ژورنال: Current Biology

سال: 1999

ISSN: 0960-9822

DOI: 10.1016/s0960-9822(00)80082-2